Coding

Part:BBa_K2250002:Design

Designed by: Shuailin Li   Group: iGEM17_Tsinghua-A   (2017-10-19)


RafD, an invertase followed by HlyA, a secretion signal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1356
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 97
    Illegal NgoMIV site found at 968
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 799


Design Notes

A GGGGS linker is added between RafD coding sequence and HlyA signal peptide so that adding of HlyA will not influence the folding and function of RafD. Also, the sequence of HlyA is synthesized directly from company, which can make it is compatible for its fusion with RafD.


Source

BBa_K2250000, BBa_K554002

References